Dia-PTT Liqiud activated partial thromoplastin time reagent.

Dia-PTT Liquid-Intended Use

Dia-PTT LIQUID is a liquid, ready to use, rabbit brain phospholipid reagent used for determination of Activated Partial Thromboplastin Time (APTT)

Dia-PTT Liquid-Test Principle

Dia-PTT LIQUID reagent initiates the activation the intrinsic coagulation pathways in the presence of standardized amount of phospholipid and contact activator (ellagic acid). After incubation,the addition of calcium induces the formation of fibrin clot. The time of this clotting process is measurable manually or with optical and mechanical coagulation analyzers.

Dia-PTT Liquid-Precautions

• Person installing the Dia-PTT LIQUID reagent must be a trained laboratory professional
• By calculating with inappropriate data or using the supplied data improperly, erroneous results may occur
• Dia-PTT LIQUID reagent, due to its ingredients should be handled with care by observing the precautions recommended for biohazards material
• Reagent coming into contact with specimens and other materials should be handled as if capable of transmitting infection and should be disposed of with proper precautions
•  Avoid microbial contamination of the reagent otherwise erroneous results may occur
• According to the present knowledge the reagent does not contain any particles which can spread from animal to human
• All reagents, waste and utilized disposable laboratory equipment should be considered as hazardous waste! Their handling and disposal should be done according to the valid hazardous material processing regulation.
• Do not use the reagent beyond the expiration date printed on the label

Dia-PTT Liquid-ACTIVE INGREDIENTS

Dia-PTT LIQUID reagent is a phospholipid from rabbit brain, which contains ellagic acid in buffered medium with preservative.

Dia-PTT Liquid-TEST PROCEDURE

Dia-PTT LIQUID test is an APTT test, which can be used with semi-automated coagulation analyzers (Coag 4D) according to the protocol detailed below. The duplicated measurement is recommended

Normal and pathological controls are recommended for verified measuring. Each laboratory should establish its own quality control program. In case of determination by any other coagulometer, please follow the instructions of the manual. Use only Dia-CaCl2 solution in order to achieve correct result.

Dia-PTT Liquid-Overview and Uses

Dia-PTT LIQUID reagent is a rabbit brain extract phospholipid. The APTT test is a sensitive screening test for the intrinsic coagulation pathway. Dia-PTT LIQUID as a reagent for APTT is highly sensitive to decreased level of factors in intrinsic pathway (factor I, II, V, VIII,IX, X, XI and XII), hereditary or acquired coagulation disorders and liver failure. Therefore, the APTT by Dia-PTT LIQUID reagent is optimally used for presurgical screening and monitoring for heparin therapy, as well. Dia-PTT LIQUID reagent with the corresponding deficient plasmas is also suitable for determination of activity of intrinsic coagulation pathway.

Dia-PTT Liquid-Storage and Stability

Dia-PTT LIQUID reagent in intact vial is stable until the expiration date given on the vial, when stored at 2-8C. Stability after opening in the original vial is shown in below table:

Dia-PTT Liquid-Expected Results 

Dia-PTT LIQUID test results can be reported in the following units, lot specific sheet in the box will help in the calculation:
1. Seconds, which means the observed clotting time.
2. Ratio (Ratio=APTT/MNPTT), which means the clotting time of the sample divided by the mean normal APTT (MNPTT). Method dependent MNPTT value in the value sheet is only for information, because it depends on the measuring circumstances and population. Every laboratory should determine its own MNPTT value and reference range. Our reference range is the following on Diagon analyzers (Coag Line):

Dia-PTT Liquid-Limitations

The result of APTT test with Dia-PTT LIQUID reagent may be influenced by drugs and other preanalytical interfering agents. The potential limits of these parameters were tested on Diagon analyzers (Coag Line) with the following result:

Dia-PTT Liquid-Performance Characteristics

The reproducibility test of Dia-PTT LIQUID reagent on Diagon analyzers (Coag Line) gives the following results:

Difference Between Dia-PT Liquid and Dia-PTT Liquid

1. Test Purpose:

• Dia-PT Liquid: Used for determining Prothrombin Time (PT), which assesses the extrinsic and common pathways of coagulation.
• Dia-PTT Liquid: Used for determining Activated Partial Thromboplastin Time (aPTT), which evaluates the intrinsic and common pathways of coagulation.

2. Coagulation Pathways:

• Dia-PT Liquid: Focuses on factors I (fibrinogen), II (prothrombin), V, VII, and X.
• Dia-PTT Liquid: Focuses on factors VIII, IX, XI, XII, and common pathway factors.

3. Clinical Applications:

• Dia-PT Liquid: Commonly used for monitoring warfarin therapy, assessing liver function, and screening for vitamin K deficiency.
• Dia-PTT Liquid: Often used to monitor heparin therapy, diagnose bleeding disorders, and evaluate clotting factor deficiencies.

4. Reagent Composition:

• Dia-PT Liquid: Contains rabbit calcium liquid thromboplastin.
• Dia-PTT Liquid: Contains activators to trigger the intrinsic pathway, such as phospholipids and a contact activator.

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