Dia-PT LIQUID prothrombin time reagent.

Dia-PT Liquid-Intended Use

Dia-PT LIQUID is a liquid, ready to use, rabbit brain thromboplastin reagent used for determination of Prothrombin Time (PT).

Dia-PT Liquid-Test Principle

Dia-PT LIQUID reagent as a calcium thromboplastin, induces the formation of fibrin clot when added to patient’s plasma. The time of this clotting process is measurable manually or with optical and mechanical coagulation analyzers

Dia-PT Liquid-Precautions

• Person installing the Dia-PT LIQUID reagent must be a trained laboratory professional.
•  By calculating with inappropriate data or using the supplied data improperly, erroneous results may occur.
• Dia-PT LIQUID reagent, due to its ingredients should be handled with care by observing the precautions recommended for biohazards material.
• Reagent coming into contact with specimens and other materials should be handled as if capable of transmitting infection and should be
  disposed of with proper precautions.
• Avoid microbial contamination of the reagent otherwise erroneous results may occur.
•  According to the present knowledge the reagent does not contain any particles which can spread from animal to human.
• All reagents, waste and utilized disposable laboratory equipment should be considered as hazardous waste! Their handling and disposal should be done according to the valid hazardous material processing regulation.
• Do not use the reagent beyond the expiration date printed on the label.   

Dia-PT Liquid-ACTIVE INGREDIENTS

Dia-PT LIQUID reagent is a tissue thromboplastin from rabbit brain, which contains calcium ions and preservative

Dia-PT Liquid-TEST PROCEDURE

Dia-PT LIQUID test is a one-stage PT test, which can be used with semi-automated coagulation analyzers (Coag 4D) according to the protocol detailed below. The duplicated measurement is recommended.

Normal and pathological controls are recommended for verified measuring. Each laboratory should establish its own quality control program. In case of determination by any other coagulometer, please follow the instructions of the manual.

Dia-PT Liquid-Overview and Uses

Dia-PT-liquid thromboplastin is a rabbit brain extract, which contains tissue factor, lipids and calcium ions. The PT according to Quick is a sensitive screening test for the extrinsic coagulation pathway. Dia-PT LIQUID as a reagent for PT is highly sensitive to vitamin K antagonists, decreased level of factors in extrinsic pathway (factor II, V, VII, and X), hereditary or acquired coagulation disorders and liver failure. Therefore, the PT by Dia-PT LIQUID is optimally used for presurgical screening and monitoring for oral anticoagulant therapy (OAT) also. Dia-PT LIQUID reagent with the corresponding deficient plasmas is also suitable for determination of activity of extrinsic coagulation pathway (factor II, V, VII and X).

Dia-PT Liquid-Storage and Stability

Dia-PT LIQUID reagent in intact vial is stable until the expiration date given on the vial, when stored at 2-8°C. Stability after opening in the original vial is shown in below table:

Dia-PT Liquid-Limitations 

The result of PT test with Dia-PT LIQUID reagent may be influenced by drugs and other pre-analytical interfering agents. The potential limits of these parameters were tested on Diagon analyzers (Coag Line) with the following result:

Dia-PT Liquid-Performance Characteristics

The reproducibility test of Dia-PT LIQUID reagent on Diagon analyzers (Coag Line) gives the following results:

Difference Between Dia-PT Liquid and Dia-PTT Liquid

1. Test Purpose:

• Dia-PT Liquid: Used for determining Prothrombin Time (PT), which assesses the extrinsic and common pathways of coagulation.
• Dia-PTT Liquid: Used for determining Activated Partial Thromboplastin Time (aPTT), which evaluates the intrinsic and common pathways of coagulation.

2. Coagulation Pathways:

• Dia-PT Liquid: Focuses on factors I (fibrinogen), II (prothrombin), V, VII, and X.
• Dia-PTT Liquid: Focuses on factors VIII, IX, XI, XII, and common pathway factors.

3. Clinical Applications:

• Dia-PT Liquid: Commonly used for monitoring warfarin therapy, assessing liver function, and screening for vitamin K deficiency.
• Dia-PTT Liquid: Often used to monitor heparin therapy, diagnose bleeding disorders, and evaluate clotting factor deficiencies.

4. Reagent Composition:

• Dia-PT Liquid: Contains rabbit calcium liquid thromboplastin.
• Dia-PTT Liquid: Contains activators to trigger the intrinsic pathway, such as phospholipids and a contact activator.

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